Table 2.
Experimental information check list. MI for each experiment is in bold, optional information is in italics.
| Experiment type |
Analyses to perform for evaluating proteins before starting the experiments |
Protocol references (from a list of accepted standard protocols for each experiment type that guarantee output congruency) |
Equipments (define minimal requisites of the devices in terms of quality, maintenance, controls) |
| Protein pull-down | SDS PAGE; AI; SEC; DLS; CD far UV; CD near UV; FT-IR; NMR; | SDS: protocol 1 (or protocol 2,..) AI protocol 1 | SDS: Hoefer minigel.... AI: Spectrofluorimeter Jasco ..... SEC: column xy and FPLC model z..... |
| Monoclonal Ab purification | SDS PAGE; AI; SEC; DLS; CD far UV; CD near UV; | ||
| Protein purification | SDS PAGE; AI; SEC;DLS; CD far UV; CD near UV; FT-IR; NMR; | ||
| SPR | |||
| ITC | |||
| ELISA | |||
| Enzymatic assay | |||
| Protein refolding | |||
| Immunoprecipitation | |||
| Immunofluorescence | |||
| Immunohistochemistry | |||
| Proximity ligation |
The corresponding pictures (SDS-gels, chromatographic profiles, fluorimeter spectra,.....) would be embedded in the metadata form.
Abbreviations: Aggregation Index (AI) (16), Size Exclusion Chromatography (SEC), Dynamic Light Scattering (DLS), Circular Dichroism (CD), Fourier-Transform InfraRed (FT-IR), Nuclear Magnetic Resonance (NMR).