Table 3.

General guidelines for good lab praxis.

Clone only in expression vectors that allow cleavage of the tag after purification. Advantage: any side effect due to the tag can be evaluated
Cleave the tag before using the target protein in interaction experiments. Advantage: unspecific interactions can be limited
Prepare mono-use aliquots of the purified protein. Advantage: reproducibility of the experiment is improved
Perform an experiment to evaluate protein stability after incubation on ice or at room temperature at different times; stability for freeze/thaw, if applicable. Advantage: experiment design is optimized