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. 2008 Jul 18;9:38. doi: 10.1186/1471-2121-9-38

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Copyright © 2008 Laval et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Role of the cytoplasmic region in the lateral mobility of paranodin analyzed by FRAP. (A) Images of neuroblastoma N2a cells co-transfected with paranodin-Δcyto (pndΔcyto) and contactin showing an example of a FRAP sequence. Bar: 5 μm. Kinetics of fluorescence recovery of GFP-tagged paranodin (B) and paranodinΔcyto (C). Data are normalized to yield an intensity of zero immediately after photobleaching and represents the average of 8 individual traces (mean ± SEM). Deletion of the cytoplasmic tail of paranodin significantly (P < 0.05) increased the mobile fraction of paranodin-GFP as assessed using ANOVA.