Figure 2.

(A) Expression of Fugu genes in transgenic rats. WT-Hypo, hypothalamus from wild-type rats; TG-Hypo, hypothalamus from transgenic rats; Brain (−Hypo), brain without hypothalamus. Adrenal glands and ovary from three rats were pooled to obtain adequate RNA for Northern analysis. RNA was fractionated on a 1.2% gel, transferred to nylon membrane (Amersham), and probed with various probes. Each lane contains approximately 20 μg (hypothalamus) or 50 μg (other tissues) of total RNA. IT and VT probes did not hybridize to RNA from the testis of wild-type rats (data not shown). (B) Effect of dehydration on levels of transgene expression. Each lane contains approximately 20 μg of total RNA from the hypothalamus of transgenic rat that was given tap water (control) or deprived of fluid for 3 days (dehydrated). A oligonucleotide probe recognizing rat α-tubulin (TUB) was used as internal standard for quantitation.