Figure 3.

In situ hybridization of brain and testis sections from transgenic rats. Tissue sections were hybridized with ³⁵S-labeled isotocin probe. OC, optic chiasma; 3V, third ventricle; S, spermatozoa. (Left) Sections were photographed under bright-field. The same sections were photographed in the dark-field (Right) to show the distribution of silver grains. IT transcripts were found only in the SON and PVN of the hypothalamus. No VT transcripts were found in any part of the brain. The IT probe did not hybridize to brain sections from the wild-type rats. The antisense oligonucleotide for IT probe did not bind to any cells in the brain or testis from transgenic rats (not shown).