Figure 4.

Double in situ hybridization analysis. Adjacent sections of brain were hybridized simultaneously with ³⁵S-labeled IT probe and digoxigenin-labeled OT probe (IT-OT) or with ³⁵S-labeled IT probe and digoxigenin-labeled VP probe (IT-VP). Silver grains represent the hybridized radioactive probe, and the gray-brown patches are the result of color reaction of the hybridized digoxigenin probe. The IT transcripts colocalize with endogenous OT transcripts in the SON and PVN, but not with the VP transcripts. OC, optic chiasma.