Table 1.

Stain	Number of images	Distance shading	Distance grey value distribution	Distance grey value height
H & E	1400	49	54	46
DAB	1200	32	39	35
AP	400	38	42	39
DAPI & TEXAS RED & FITC	120	98	75	129

H & E: hematoxilin – eosin; DAB: diaminobencidine; AP: Alcalic phosphatase; DAPI & Texas Red & FITC: tri-colour fluorescent stain