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Explanations: H & E: hematoxilin – eosin; DAB: diaminobencidine; AP: Alcalic phosphatase; DAPI & Texas Red & FITC: tri-colour fluorescent stain
| Stain | Number of thresholds | Area covered first threshold | Area covered 2nd threshold |
| H & E | 3 | 54% | 10% |
| DAB | 3 | 63% | 12% |
| AP | 3 | 45% | 11% |
| (DAPI & TEXAS RED & FITC | 2 | 35% | 5% |
