Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Aug 11;182(3):509–517. doi: 10.1083/jcb.200801079

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Yu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Cyclin D1 induces miR-17-5p and miR-20a expression. (A) Northern blotting showed the increased expression of miR-17-5p and miR-20a in the MSCV–cyclin D1–infected MEF cells. miR-100 was used as a negative control. tRNA served as loading control. (B) Breast cancer cell line MCF-7. (C) cyclin D1^−/−, and cyclin D1^+/+ MEFs were starved by 5% charcoal-stripped serum for 48 h, followed by 10% FBS stimulation. The time course examination of the cyclin D1 protein level and the miR-17-5p expression level were performed by Western and Northern blots, respectively. The results showed the induction of cyclin D1 and miR-17-5p by serum stimulation in the MCF-7 and cyclin D1^+/+ MEF cells, but not in cyclin D1^−/− MEFs. (D) miR-17-5p and miR-20a detection in the MMTV–cyclin D1 transgene induced mammary tumors (n = 3) compared with normal mice mammary gland. Data from three mammary tumor samples were presented as mean ± SEM. (E) Real-time PCR analysis of cyclin D1 chromatin immunoprecipitated DNA. 11 fragments derived from chromosome 11q31 upstream of miR-17/20 cluster were examined. For each fragment, amplifications on chromatin before immunoprecipitation and chromatin immunoprecipitated with preimmune serum were performed as input and negative control, respectively. This experiment was repeated three times; data are equal to mean ± SEM.

HHS Vulnerability Disclosure