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T-synthase expressed in LSC Cells is partially ubiquitinated. LSC and LSB cells stably expressing T-synthase–HPC4 were treated with MG-132 for 12 h and subsequently harvested. T-synthase from 200 μl of cell extract (500 μg of protein total) was purified and analyzed by Western blot with anti-ubiquitin (A) first, and then with anti-HPC4 (B) after stripping of the membrane.
