Table I. PCR primers used for making expression constructs.

Constructs	Forward primer	Reverse primer
Cosmc-HA	5′-GCGGATCCACCATGCTTTCTGAAAGCAGC     TCCTTTTTG-3′	5′-GCTCTAGACTAAGCGTAGTCTGGGACGTCGTATGGGTAG     TCATTGTCAGAACCATTTG-3′ (containing HA-tag sequence)
HPC4-Cosmc	5′-GCGGATCCACCATGCTTGAGGACCAGGTGGA     CCCCAGGCTGATCGACGGCAAGATGCTTTCTGA     AAGCAGCTCC-3′ (containing HPC4-tag sequence)	5′-GGTCTCCAGATTTTATAGTGTGGC-3′
HPC4-sCosmc-KDEL	5′-TCCTTGCACGCCCCACTACG-3′	5′-GCTCTAGAGTTCATCTTTGTCATTGTCAGAACCATTTG-3′     (containing KDEL sequence)