Figure 4.

CGH-1 associates with specific maternal mRNAs. (A) Identification of CGH-1–associated mRNAs by RIP-Chip. CGH-1 was immunoprecipitated from extracts from 1-d-old adult hermaphrodites, then mRNA was extracted from material that was eluted by the immunogenic CGH-1 peptide, a protocol that isolates the CGH-1 complex (Boag et al., 2005). The control was rabbit IgG. After linear amplification, samples were labeled with Cy3 or Cy5 and hybridized to microarrays. Fold enrichment was averaged from four RIP-Chip experiments. (B) Expression profiles of CGH-1–enriched mRNAs. The vast majority of annotated CGH-1–enriched mRNAs are expressed primarily in adult hermaphrodite gonads, which produce only oocytes (Reinke et al., 2000, 2004). (C) CoIP with CGH-1 does not correlate with abundance. The relative enrichment in CGH-1 IPs and the number of nonambiguous SAGE tags (normalized to 100,000) are plotted for the 50 mRNAs that were most enriched in CGH-1 IPs (blue) and the 50 most abundant mRNAs (red) from a dissected gonad SAGE library (SW040; http://tock.bcgsc.bc.ca/cgi-bin/sage140).