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. 2008 Aug 11;182(3):573–585. doi: 10.1083/jcb.200802164

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© 2008 Han et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — High K⁺ treatment regulates Drp1 dynamics in HeLa cells. (A) HeLa cells were transfected with control siRNA (siCont) and siRNA for Drp1 (siDrp1) before the transfection with GFP, YFP-Drp1^R, or YFP-Drp1^R-S600A and mito-DsRed as described above. HeLa cells were then either untreated or incubated with 20 mM K⁺ for 15 min. (B) Quantitative analysis of exogenous Drp1 foci. Data were obtained from the results from at least five independent experiments for each condition shown in A. Values were analyzed using one-way ANOVA followed by post-Turkey test. Control is siDrp1 without stimulation. Error bars indicate SD in each group. *, P < 0.01 versus siDrp1+YFP-Drp1^R+No stimulation. Bars, 10 μm.