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. 2008 Aug 11;182(3):587–601. doi: 10.1083/jcb.200709177

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© 2008 Noda et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — TM5b overexpression inhibits AQP2 trafficking in MDCK/AQP2 cells. (A) MDCK/AQP2 cells were transfected with GFP-TM5b or GFP (green) and treated without (top) or with (bottom) forskolin. Cells were labeled for AQP2 (red). Bars, 10 μm. (B and C) Apical cell surface biotinylation assay using MDCK/AQP2 cells transfected with GFP-TM5b or GFP, corresponding to A. (B) Biotinylated proteins were precipitated with streptavidin-agarose beads and immunoblotted for AQP2. (C) The densitometric quantification normalized to GFP-transfected cells without forskolin stimulation. Data represent the mean and SE from three independent experiments. *, P < 0.05 versus GFP-transfected cells without forskolin stimulation; **, P < 0.05 versus GFP-transfected cells with forskolin stimulation.