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. 2008 Aug 11;182(3):603–614. doi: 10.1083/jcb.200705102

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© 2008 Veracini et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — PAG-N does not regulate growth factor signaling outside caveolae. (A) PAG-N does not affect PDGF-induced dorsal ruffles' formation. (A, left) An example of circular dorsal ruffle formation assessed by actin staining in NIH 3T3 cells infected with the indicated retroviruses, stimulated or not with PDGF for 10 min. (A, right) Statistical analysis of dorsal ruffle formation in cells infected with the shown retroviruses, treated, or not, with 5 μM SU6656 for 30 min and stimulated with PDGF for 10 min. (B) EGF-induced mitogenic response is not regulated by caveolae in NIH 3T3 cells. Quiescent cells transfected with Cav3-DGV were stimulated with 25 ng/ml PDGF or 100 ng/ml EGF in the presence of BrdU. (C) PAG-N does not affect EGF-induced DNA synthesis. The percentage of transfected (or infected) cells that incorporated BrdU or that formed dorsal ruffles was calculated as described in Material and methods. Results are expressed as the mean ± SD of three to five independent experiments. **, P < 0.01 (using Student's t test).