Figure 2.

Confocal investigations of treated and untreated human peripheral lymphocytes. The green fluorescence signals (2b) originate from both the biotinylated-CAI-BioShuttle and intrinsic biotin after treatment with Streptavidin, Alexa Fluor® 488-solution. A strong red fluorescence signal of the mitochondrial staining by the used MitoTracker red is detectable. The overlay of the figures 2a and 2b as well as the corresponding DIC picture (2d) shows that the green fluorescence signal of the CAI-BioShuttle is co-localized with the red fluorescence of the mitochondrial compartment resulting in orange (mix fluorescence (2c). The bars indicate 20 µm.