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. 2008 Aug 20;3(8):e2983. doi: 10.1371/journal.pone.0002983

Figure 3. Expression of S. oneidensis ORFs from the pTP248 double-tag destination plasmid.

Figure 3

A. The protein expression levels of ORFs were evaluated from whole cell protein lysates of E. coli by SDS-Page fractionation and immunodetection using anti-GST antibody. The S. oneidensis ORF number is shown above each lane. The red asterisks indicate the position of a band that is consistent with the predicted molecular weight of the fusion protein. Note that for ORFs SO0047 and SO0056, a band is not visible in panel A but is visible in the longer film exposure of panel B. The predicted molecular weights (kDa) of the fusion proteins are: SO0037, 40.7; SO0038, 65.1; SO0040, 61.2; SO0041, 41.4; SO0044, 39.7; SO0047, 75.1; SO0049, 86.9; SO0051, 34.3; SO0053, 66.8; SO0054, 74.2; SO0055, 94.7; SO0056, 59.3; SO0057, 79.9; SO0059, 57.1. B. Identical to A. but with a longer film exposure.