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. 2008 Aug 20;3(8):e2983. doi: 10.1371/journal.pone.0002983

Figure 5. In vitro biopanning protocol.

Figure 5

Briefly, phages were incubated with immobilized anti-E. coli thioredoxin antibody, non-binders were removed by washing, bound phage were eluted, amplified, and the process was repeated. Eluted phage clones from round 3 were DNA sequenced.