Figure 2. Expression confirmation of novel miRNAs identified by cloning from specific development stages of silkworm using stem-loop RT PCR.

For bmo-miR-2008, we detected its three different mature segments, bmo-miR-2008a, bmo-miR-2008b and bmo-miR-2008c; for bmo-miR-2009, we detected only the mature segment from 5′ arm of its precursor. U6 snRNA serves as a positive control.