Table 2. Cell number and percentage of uni-nucleated, bi-nucleated and multi-nucleated cells during cell division of E. histolytica in axenic cultures.
| Time (h) | (No. of cells±S.D.)×104/ml (n = 3) | Percentage of nuclei per cell±S.D. (n = 3) | ||
| 1 | 2 | >2 | ||
| 0 | 6.0±0.0 | 84.7±0.8 | 12.5±0.5 | 2.5±0.5 |
| 12 | 10.5±0.5 | 86.4±0.4 | 11.5±1.5 | 1.9±0.9 |
| 24 | 17.5±1 | 90.9±1.2 | 7.5±0.5 | 1.2±0.2 |
| 36 | 29.6±0.9 | 87.0±1.0 | 11.6±0.6 | 1.6±0.6 |
| 48 | 56.2±2.2 | 88.3±1.3 | 9.4±0.4 | 1.8±0.8 |
| 60 | 90.5±4.5 | 89.5±0.5 | 8.7±0.3 | 1.7±0.7 |
| 72 | 200.1±9.4 | 89.1±1.1 | 9.3±0.7 | 1.8±0.2 |
Cell numbers were counted for the CFSE labeled and unlabelled cultures at the indicated time points. The number of nuclei in each cell was counted manually in at least 500 cells after staining with DAPI, and the percentage of uni-nucleate, bi-nucleate and multi-nucleated cells was calculated.