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. 2008 Aug 20;3(8):e3007. doi: 10.1371/journal.pone.0003007

Figure 1. PAC detection of an exon-skipping PTEN mutant.

Figure 1

(A) Normalized expression data of all exons within the PTEN gene. Each exon probe set is represented by a dot in the solid line; multiple probe sets may be directed against the same exon. (B) PAC normalizes the variability in gene expression levels between samples and, in a single sample, the variability in signal intensity between probe sets of the same transcript. PAC calculation therefore allows rapid detection of skipping of PTEN exon 4 in breast cancer cell line MDA-MB-468 due to a PTEN c.253+1G>T splice site mutation that we previously had identified [17].