Figure 5. Identification of nucleotide substitutions by PAC.

(A) PAC predicts skipping of the 5′ end of PTEN exon 5 in the CAMA-1 breast cancer cell line. This cell line contains a nucleotide substitution within the identified exon. This base change does not induce exon skipping but is centrally located within all three probes of the probe set (B). The central location suggests this mutation causes a reduced affinity to the probes on the exon-array.