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. 2008 Aug 20;3(8):e3007. doi: 10.1371/journal.pone.0003007

Figure 6. PAC Identification of novel genetic changes.

Figure 6

(A) PAC detection of novel genetic changes in EGFR. PAC predicted skipping of the last four exons of GBM157 and the 5′ end of exon 17 in GBM172. Semi-quantitative PCR on genomic DNA confirmed the deletion in GBM157 (not shown). (B) PAC predicts skipping of exon 30 in the FCGBP gene in GBM60. (C) RT-PCR confirmed the FCGBP exon skipping event in GBM60; other tumors did not show this exon skipping. (D) Direct sequencing identified a single base change in EGFR in GBM172 (as predicted by PAC, see Fig. 6A). (E) Confirmation of a PAC predicted change in the TLE2 gene in GBM60. The nucleotide substitution overlaps with individual probes of the probe set.