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. 1997 Dec 23;94(26):14412–14417. doi: 10.1073/pnas.94.26.14412

Figure 2.

Figure 2

RPR120535 and RPR120535/DNA complexes from zones A, B, and C (see Fig. 3), visualized by light microscopy and transmission electron microscopy (TEM). (A) Cryo-TEM micrographs of an aqueous solution of RPR120535 alone. (B) Electron micrograph of uranyl acetate-stained complexes from zone A (RPR120535/DNA charge ratio: 0.3). Inset shows the same complexes at higher magnification. (C) Complexes from zone B (charge ratio: 1.65) observed by light microscopy. (D) Electron micrograph of the same complexes stained with uranyl acetate. (E) Cryo-TEM micrograph of RPR120535/DNA complexes originating from zone C (charge ratio: 6). Inset shows the visualization by cryo-TEM of the ordered domains in these complexes. (F) Electron micrograph of uranyl acetate-stained complexes from zone C. (G) Cryo-phosphotungstate-TEM micrograph of the same complexes. In this micrograph, the complexes seem to have aggregated, because the thickest part of the vitrified film allows them to be superimposed. The scale bar represents 100 nm in A, B Inset, and D–G; 500 nm in B; and 10 μm in C.