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. 1997 Dec 23;94(26):14412–14417. doi: 10.1073/pnas.94.26.14412

Figure 3.

Figure 3

(A) Colloidal stability of RPR120535/DNA complexes, at 0.1 mg DNA/ml, as a function of the RPR120535/DNA charge ratio (ratios were raised from 0 to 7 by increments of 0.3), in 4, 40, 80, or 150 mM NaCl. RPR120535/DNA complexes were defined as colloidally stable if their mean diameter was below 700 nm for at least 24 hr. Complexes from zones A and C were stable, whereas those from zone B exhibited a visible precipitate. (B) In vitro luciferase activity, measured in transfected HeLa cells, of RPR120535/DNA complexes originating from zones A, B, and C. Inset shows, in transfected NIH 3T3 cells, the in vitro transfection activity of DNA complexes with RPR120535 micelles or liposomes, i.e., RPR120535/DOPE (1/1, M/M), both at a charge ratio of 6, and of DNA complexes with DMRIE/Cholesterol, at a molar ratio of 1.65.