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Chemical structure of N4-acetylcytidine (ac4C) and secondary structure of E. coli elongator tRNAMet. (A) Chemical structure of ac4C. (B) Secondary structure of E. coli elongator tRNAMet with modified nucleosides: 4-thiouridine (s4U), 2′-O-methylguanosine (Gm), dihydrouridine (D), N4-acetylcytidine (ac4C), N6-threonylcarbamoyladenosine (t6A), pseudouridine (Ψ), 7-methylguanosine (m7G), 3-(3-amino-3-carboxypropyl) uridine (acp3U) and 5-methyluridine (m5U).
