Table 3. Clinical signs, virus replication and seroconversion in direct and aerosol contact ferrets.
Virus | Direct contact ferrets | Aerosol contact ferrets | ||||
Virus detected in nasal washa | Sneezing (Day of onset) | Serum (HI titer) b | Virus detected in nasal washa | Sneezing (Day of onset) | Serum (HI titer) b | |
RGWF10c | 3/3 | 0/3 | 320, 320, 640 | NDd | ND | ND |
RGWF10 | 2/2 | 0/2 | 640, 1280 | 0/2 | 0/2 | <10, <10 |
Dk/HK/Y280/97 | 1/2 | 0/2 | 640, <10 | ND | ND | ND |
RGQa88 | 0/2 | 0/2 | <10, <10 | ND | ND | ND |
Ck/HK/SF3/99 | 0/2 | 0/2 | <10, <10 | ND | ND | ND |
Ck/Jordan/554/03 | 0/2 | 0/2 | <10, <10 | ND | ND | ND |
RGMemphis98 | 2/2 | 2/2 (4, 4) | 5120, 5120 | 2/2 | 2/2 (8,10) | 2560, 5120 |
2WF10:6M98 | 2/2 | 2/2 (4, 5) | 1280, 1280 | 0/2 | 0/2 | <10, <10 |
Virus in nasal washes was analyzed using FLU DETECTâ„¢ Antigen Capture Test Strip (Synbiotics Corp.) and titrated by TCID50.
Homologous virus was used in the HI assays to detect anti-H9 antibodies.
For the RGWF10 virus, two separate experiments were performed.
ND, not done.