Figure 4. Alignment of insect SPS1 and SPS2 proteins.

The black arrow shows the position of the selenocysteine residue in SPS2 and Arganine or Cysteine in SPS1. In A. mellifera, we use “?” to denote the codon TGA. Although we believe that in this case, TGA is being readthrough to incorporate Arginine (R), it actually aligns with the Sec-incorporating codon in SPS2.