Table II.

Comparison of calculated and experimental mass with that identified from the 2D gel analysis

The calculated mass was obtained based on the amino acid sequence in GenBank™. The experimental mass was taken from the results of data from the literature and company data sheets.

Protein	Calculated mass	Experimental mass	Mass on 2D gel
	kDa	kDa	kDa
Cytoskeleton and cytoskeleton-associated
Drebrin 1 isoform a	71.9	116/125	125/132/134/131
β-Actin	42	42	46/32
Spinophilin	89.6	120–140	131
α-Actinin-1	103	107	123
α-Actinin-4	105	100–115	120, 123
Capping protein α	33	34	35
Ezrin	69.4	81	100
Cofilin I	18.7	19–21	13.25
Glial fibrillary acidic protein	49.5	50	47
α-Tubulin	50	57	75
Signaling
14-3-3ζ	31	30	26/26/34/32/32/31
14-3-3ε	28	31	30
Inhibitor-2 of protein phosphatase 2A	31–33	30	48/48/46
Chaperones
Hsp90α	85.1	90	113/111
Heat shock protein gp96 precursor	92.7, 90.3	100	125/122
Valosin-containing protein	90	97	118
Protein translation and conjugation
Eukaryotic elongation factor 1γ	50.5	46	58
Metabolism
Inorganic pyrophosphatase	33.1	33	35
Glyceraldehyde-3-phosphate dehydrogenase	36.2	38	36, 38
Proteases
Neurolysin (EC 3.4.24.16)	81.3	80	96
Prolyl endopeptidase (prolyl oligopeptidase, EC 3.4.21.26)	81.6	79.6	96
Membrane and lipid binding
Rab GDP dissociation factor inhibitor α	51	50	78
Chain A, crystal structure of brain fatty acid-binding protein oleic acid	14.8	13–14	11
Annexin V	35.8	38	38