Figure 1.
The two-bait interaction trap. Cells contain bait1, a protein fusion of Tet repressor and protein X, and bait2, a fusion of LexA and protein Y. In cell 1, a prey, a fusion of protein Z1 and transcriptional activator B42, does not interact with either X or Y. The reporters are not activated, the cell grows on 5-FOA, does not grow on Ura− medium, and is white on X-Gal. In cell 2, another prey, a fusion of Z2 and B42 interacts with X but not with Y, the TetOp-URA3 reporter is selectively expressed, and the cell grows on Ura− medium but is white on X-Gal.