Table 2.
Examples of cells that register the And and discrimination relationships (Ls1 and Ls2) on the touching relationships A1 and A2
| Baits | Prey | Reporter output
|
Logical relationship | |
|---|---|---|---|---|
| Ura- | X-Gal | |||
| 1 TetR-c-Raf1 | Ras | Growth | Blue | And |
| LexA-hSos1 | ||||
| 2 TetR-RasV12 | c-Raf1 | Growth | White | Ls2 |
| LexA-Max | ||||
| 3 TetR-RasV12 | Mxi1 | No growth | Blue | Ls1 |
| LexA-Max | ||||
| 4 TetR-RasA15 | c-Raf1 | No growth | Blue | Ls1 |
| LexA-RasV12 | ||||
| 5 TetR-RasA15 | Cdc25 | Growth | White | Ls2 |
| LexA-RasV12 | ||||
Cells were grown on raffinose medium, on which preys are expressed. Identities of the bait and prey proteins are given in the first two column headings. Reporter output phenotype is indicated by growth or blue color on the indicated medium. The logical relationship registered is given in the last column.