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Cellular localization of exogenous PTEN in transfected cells. U87, U178, and LN229 cells were seeded on coverslips and transfected with the indicated plasmids. Expressed HA-epitope-tagged PTEN was detected by immunofluorescence with anti-HA, followed by staining with a fluorescein-conjugated secondary anti-IgG1a antibody. Specificity of the primary antibody is indicated by the lack of staining of untagged PTEN.
