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. 1997 Nov 11;94(23):12479–12484. doi: 10.1073/pnas.94.23.12479

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Copyright © 1997, The National Academy of Sciences of the USA

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Growth suppressive effect of PTEN alleles in human glioma cell lines. Empty vector (pBP), or vector containing wild-type PTEN, PTEN-HA, or mutant alleles tagged with HA (G129R, d55/70, d237/239) were transfected into (A) U87 and (B) U178 (both mutant PTEN backgrounds), and (C) LN229 (wild-type PTEN background) and then selected with puromycin. Viable cells were counted after 7 days. Results are normalized in terms of percentage of vector transfection with the vector control set to 100% in each case. A typical transfection is shown. Results were reproduced in three independent experiments.