Table 1.

Summary of PTEN mutations in glioma cell lines

Cell line	Codon	Mutation	Predicted effect	Detected by
LN18		None	None	PTT/seq
LN215	105	TGT to TTT	Cys to Phe	PTT/seq
LN229		None	None	PTT/seq
LN235		ND	No expression	Northern/RT-PCR
LNZ308		Splice variant/del exon 6	Frameshift	RT-PCR
LN319	15	AGA to ATA	Arg to Ile	Seq
LN340	15	AGA to AGT	Arg to Ser	Seq
LN428		None	None	Seq
LN443		Splice variant/del exon 5	Frameshift	PTT/seq
LN444		Splice variant/del exon 5	Frameshift	PTT/seq
LN464	183	AAG to AAAG	Frameshift	PTT/seq
SF763MG		None	None	PTT/seq
T98G	42	CTT to CGT	Gly to Gln	Seq*
U87		Splice variant/del exon 3	In-frame deletion	PTT, seq*
U178	248	CCT to ACCT	Frameshift	PTT/seq
U251	242	TTT to TTTT	Frameshift	PTT, seq*
U373	242	TTT to TTTT	Frameshift	PTT, seq*
D247		Splice variant/del exon 4	Frameshift	PTT/seq

PTEN mutations were detected by a combination of Northern blot analysis, RT-PCR, and cDNA sequencing. del, deletion; seq, sequenced. 

^*Previously published data (9, 10).