Figure 2.

Interaction of Rapgap1 with Rap1 in vitro and in vivo. (A) Hydrolysis of GTP by GST–Rap1 in the presence of GST–Rapgap1. Control experiments measuring hydrolysis in the presence of GST alone or in the reaction buffer are shown. (B) Flies expressing three copies of the GMR–Rapgap1 transgene. (C) Flies heterozygous for a loss of function mutation in Rap1. (D) Flies carrying three copies of the GMR-Rapgap1 transgene that are also heterozygous for a loss-of-function mutation in Rap1.