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. Author manuscript; available in PMC: 2008 Aug 7.
Published in final edited form as: J Gen Virol. 2007 Jan;88(Pt 1):61–70. doi: 10.1099/vir.0.82216-0

Fig. 4.

Fig. 4

Induction of IFN-γ-producing CD8+ T-cell responses by repeated immunization with free or TMPEG/liposome-complexed MVAIIIB/β-gal in BALB/c mice. BALB/c mice (n=4) were immunized with an unconjugated MVAIIIB/β-gal viral vector (vertically hatched bars), TMPEG/liposome-complexed MVAIIIB/β-gal (diagonally hatched bars) or TMPEG/liposomes and MVAIIIB/β-gal delivered separately (filled bars). Control mice (empty bars) were immunized with the wild-type MVA and TMPEG/liposomes. Booster immunizations were carried out every 4 weeks. The frequencies of CD8+ T cells secreting IFN-γ in cultures established from spleen (a) and Peyer’s patches (b) were determined by ELISPOT assay with the Env-specific peptide l10. Results are shown as the means±SD of at least three independent experiments. The frequencies of cells secreting IFN-γ were determined by regression analysis from curves generated by plotting numbers of spots versus effector cells. *, P<0.05; **, P<0.001.