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. 1997 Dec 23;94(26):14438–14443. doi: 10.1073/pnas.94.26.14438

Figure 2.

Figure 2

Figure 2

Figure 2

Comparisons of the predicted amino acid sequences of mammalian exocyst subunits with respective Caenorhabditis elegans and yeast homologs. Predicted amino acid sequences of rexo70, rsec15, and rsec5 derived from rat cDNA analyses are compared with the homologous sequences in the database with the pileup program of GCG. Identical residues are in a black box with white letters and similar residues are shaded. Dotted regions represent gaps. Lines above the amino acid sequences indicate the peptides determined by amino acid sequencing of the subunits of the purified mammalian complex. (A) rexo70: Accession numbers of C. elegans and yeast homologs are U80437 and P19658. The smallest sum probability for the homology between the C. elegans and rat exo70 is 2.6 × 10−24. pileup analysis indicates that rat and C. elegans share 25% identity and 42% similarity, rat and yeast share 19% identity and 34% similarity, and C. elegans and yeast share 17% identity and 30% similarity. (B) rsec15: Accession numbers of C. elegans and yeast homologs are U41026 and Z72755, respectively. The smallest sum probability for the homology between the C. elegans and rat exo70 is 2.2 × 10−153. pileup analysis indicates that rat and C. elegans share 36% identity and 50% similarity, rat and yeast share 18% identity and 34% similarity, and C. elegans and yeast share 16% identity and 30% similarity. An asterisk (∗) at the end of the amino acid sequences of C. elegans homolog indicates the first possible stop codon in the genomic sequence. (C) rsec5: Accession numbers of C. elegans and yeast homologs are Z68319 and Z50046. The smallest sum probability for the homology between the C. elegans and rat exo70 is 3.2 × 10−61. pileup analysis indicates that rat and C. elegans share 28% identity and 46% similarity, rat and yeast share 18% identity and 31% similarity, and C. elegans and yeast share 13% identity and 27% similarity. (D) exo84: The 716 amino acid protein contains the sequences of seven peptides determined from amino acid sequencing of the 84-kDa band. The peptide sequences are underlined.