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. 1997 Nov 11;94(23):12497–12502. doi: 10.1073/pnas.94.23.12497

Figure 6.

Figure 6

Lethal effect of expression of an rnhA+ gene on rnhA224 PmioC112 oriC207::bla Pgid-103 mutant. Cells were transformed with pRNH-Km (carries lacZ::rnhA gene fusion) or pHK (vector) and selected for kanamycin resistance. Transformants were then transformed with pLacIq, selecting for tetracycline resistance. Equal amounts of cells were spread on minimal plates with or without 5 mM IPTG as indicated. (A) rnhA224 PmioC112 Pgid-103/pHK, pLacIq. (B) rnhA224 PmioC112 Pgid-103/pRNH-Km, pLacIq. (C) rnhA224 oriC207::bla PmioC112 Pgid-103/pHK, pLacIq. (D) rnhA224 oriC207::bla PmioC112 Pgid-103/pRNH-Km, pLacIq.