Table 1.

Increase in Ins(1,4,5,6)P₄ levels after Salmonella infection of T₈₄ intestinal epithelial cells

Bacteria added	Time after infection, min	[3H]Ins(1,4,5,6)P4 levels, ratio infected/control	n
S. dublin lane	30	13.9 ± 0.8	4
S. dublin lane	60	10.3 ± 1.3	5
S. dublin lane	120	3.6	2
S. dublin lane	180	1.9	2
S. typhi BRD691	30	10.5	2
S. dublin SB133 (invA)	30	1.9 ± 0.1	3
S. flexneri	60	1.9	2
S. dysenteriae	60	2.3	2
Y. enterocolitica	60	2.6 ± 0.1	3
E. coli O29:NM	60	2.0 ± 0.1	5
E. coli O157	60	2.3 ± 0.2	4
E. coli DH5α	60	2.1 ± 0.2	4
LPS	60	1.0	2

[³H]Inositol-labeled T₈₄ monolayers were infected with various bacteria for the indicated times. [³H]Ins(1,4,5,6)P₄ levels were determined by HPLC and are expressed as ratio of the level in infected cells to that in control cells. Results are means ± SEM of the number of determinations indicated in the last column. LPS (lipopolysaccharide) from E. coli O111 was used at 10 μg/ml.