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. 1997 Nov 11;94(23):12509–12514. doi: 10.1073/pnas.94.23.12509

Figure 5.

Figure 5

DNA methylation analysis of region spanning exons u1 and u2. (A) Probe B on a Southern blot of BamHI/SmaI digested DNA from samples as indicated. Polymorphic BamHI sites and hybridization signals denoted by subscripts in parentheses: d, M. domesticus; s, M. spretus. Normal vs. disomy analysis: 2.4-kb and 0.9-kb fragments in normal, but not matDi7 placenta, indicates relative demethylation of paternal allele. Maternal deletion of H19 in (M. domesticus × M. spretus)F1 samples: relative demethylation of paternal (M. spretus) allele in placenta indicated by reduced intensity of 3.7-kb, and appearance of 3.5-kb, bands. Demethylation of maternal (M. domesticus) allele in placenta in response to H19 deletion indicated by appearance of 2.4-kb and 0.9-kb bands. (B) Summary of analysis of fetus and placenta from normal and maternal H19-deficient midgestation embryos. Probes: A, XbaI–EcoRI; B, XbaI–XbaI; C, BamHI–EcoRI fragments. H1–H5, HpaII clusters; Sm, SmaI. Mat, maternal allele; Pat, paternal allele. Filled or partially filled circles indicate degree of methylation of sites. H19 deletion has no effect on fetus. H4 is fully methylated in all samples.