Figure 6.

Effects of C-PKA depletion from cytosol on vesicle release. (A) 45 μg of cytosolic proteins from both native bovine brain cytosol (control) and cytosol incubated with cAMP-agarose (depleted) were processed by SDS/PAGE and their content in C-PKA determined by Western blot analysis. (B) The vesicle release assay was performed in the presence of either native cytosol (control), C-PKA-depleted cytosol (depleted), or C-PKA-depleted cytosol supplemented with 8 units C-PKA. VSV-G immunoprecipitated from released, lysed vesicles is shown.