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Amino acid alterations in Rap1 and Ras1 mutant proteins. The amino acid sequences of Drosophila Rap1 (15) and Ras1 (12) are shown. The GTP-binding regions are boxed (21) and the region thought to bind effector is underlined (22, 23). The amino acid changes in Rap1R (15), Rap1V153M, Rap1T58M, and Ras1E63K mutant proteins are indicated. The codon changes are V153M (GTG → ATG), T58M (ACG → ATG), and E63K (GAG → AAG).
