Figure 5.

CDC45 and CDC7 are dependent on one another for function. (A) A cdc45-1 cdc7-4 culture was arrested at 38°C, the restrictive temperature for cdc7-4. At 0 h, the culture was released into medium at 11°C, the restrictive temperature for cdc45-1. Samples were monitored by flow cytometry. (B) Cells were arrested in medium at 38°C. At 0 min, the culture was released at 27°C, the permissive temperature for both cdc45-1 and cdc7-4. (C) Cells were arrested in medium at 11°C. At 0 min, the culture was released at 38°C. (D) Cells were arrested in medium at 11°C. At 0 min, the culture was released at 27°C.