Table 1.

Summary of the cellular distribution of wild-type and mutant CD-MPR

Construct	% of surface*	Gradient, % in lysosomes†	% of internal pool in lysosomes‡
ML4 (wild-type) (CD-MPR)	17  ±  5 (n = 4)	4  ±  1	5
F13A	6  ±  2 (n = 3)	9  ±  4 (n = 5)	10
F18A	33  ±  8 (n = 3)	13  ±  2 (n = 5)	19
F32A	16 ± 2§	2  ±  1 (n = 3)	2
W19A	24  ±  6 (n = 7)	33  ±  10 (n = 7)	43
FW→A	48  ±  2 (n = 3)	29  ±  8 (n = 5)	56
FFW→A	56  ±  3 (n = 3)	29  ±  8 (n = 7)	66
FFWY→A	67  ±  5 (n = 5)	22  ±  4 (n = 5)	67
MPR-FF	29  ±  19 (n = 9)	5  ±  3 (n = 4)	7
MPR-FY	18  ±  7 (n = 9)	4  ±  2 (n = 3)	5
MPR-YF	27  ±  18 (n = 9)	8  ±  5 (n = 7)	11
MPR-YW	18  ±  8 (n = 9)	3  ±  2 (n = 5)	4

^*Percentage of the various mutant receptors that were present at the cell surface at steady state. The values are expressed as mean ± SE; n is the number of determinations. 

^†Percentage of the various mutant receptors recovered in dense lysosomes on Percoll gradients as described in Fig. 1. The values are expressed as mean ± SE; n is the number of determinations. 

^‡The percentage of the internal pool of receptor that is in lysosomes was calculated using the numbers for the percentage on the surface and in lysosomes. 

^§Taken from ref. 4.