Figure 4.

T. parva infection mediates continuous degradation of IκBα and IκBβ. T. parva infected T cells (−) or the same cloned cell line cleared of the parasite using BW720C (+) were treated with cycloheximide (CHX) to inhibit protein synthesis. The duration of CHX treatment in hr (h) prior to cell harvest is designated at the top of each figure. Okadaic acid (OA), which blocks the serine/threonine phosphatase inhibitors types PP1 and PP2A, was included as a positive control for IκBα degradation. IκBα (a) and IκBβ levels (b) were determined by immunoblot analysis.