Table 2. Primers used for the mutation analysis of the SLC4A11 gene.

Exon	Sequence (5'-3')	Annealing temperature (°C)	Amplicon size (bp)
2	F: ATTAAGGCTGGCTTCCCCTGCTATG	67	303
	R: CCCTGGAGGCTTTTGCCCGACAAG
3&4	F: CCTTCCTGTGTGTGGCACTTTAACAG	67	439
	R: ATCACCTCAGCCCCCAGGTAGAGG
5	F: ACCAGGCAGTGACAGCATCTCATAC	67	354
	R: GGGTGGTGGGTCAACAGCCCCTC
6	F: GGGGGCGTTGGGAGGGGCTGTTGA	67	201
	R: AGGGGACATGGGACACCCAGTTCCAC
7	F: GTCGGGGAGCCCCAGCTCCCTGG	67	254
	R: GGACCCCAAGCAGAGGGCGGGTAA
8	F: CCCGGGCAGGGCCTCCTCTGTTTC	67	328
	R: GACAGAGCGCCTGTTAGCCCTGTCC
9&10	F: TCGGCGGGGGCATGGGCCGGACA	67	419
	R: AGCCCAGGGCCCAGCCCAGCATAC
11	F: TATGCTGGGCTGGGCCCTGGGCTG	67	444
	R: GGGCTGAACCAGATCCCAAGCCTTGA
12	F: GGGGCTCAGGGAGGCCTCCCCCA	67	210
	R: AGTGCAGAACCTCCCATCTCGGCTG
13	F: TCCAGGGCCTCCCCCTGCCACAC	67	382
	R: GGGACAGCAGGTGCATGAGCACAGC
14	F: GAGGGGAGGGGCCGCATGGGTCAA	67	239
	R: AGTAGGGGACAGGCTACTGCTATGCC
15	F: GGCGGTGGGTGACGTGGGGTAGC	67	304
	R: CTCGTGGACAGAGCCCCACAGCAGA
16	F: CACCGGAGAACAGGTGTGGAGGGTG	65	325
	R: GGCCAGAGGCTCCCCACTCCTCAG
17	F: GGAGGAGTGAGGCCCTGTGGACAGG	67	340
	R: TGTGGGCGGCAGGGACCGGGTGTG
18	F: GGCGTGGGTGGGGACACAGCCCCA	67	289
	R: CAGCCCGCCCATTCTCCACACCTAGA
19	F: TGGGATGGGTGTCCACTGCCTTCTC	67	199
	R: GCTCCAGAGCCAGCCTGGGAGGAC

Primers were designed using genomic DNA and mRNA (GenBank NM_032034) sequences from the UCSC Genome Bioinformatics site. This mRNA does not have exon 1 sequences. Exon 1 was not analyzed as it is a part of the 5'-UTR.