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Ca2+ mobilization in 300-19 cells expressing CCR2B and Gαt. Stably transfected 300-19 cells were loaded with Indo-1 AM, and intracellular calcium levels in response to 100 nM MCP-1 (A) or to the indicated concentrations of MCP-1 (B) were measured as described in Fig. 1. Data are representative of three independent experiments.
