Table 1.
Expression of surface molecules on HLA-DR+lin−IL-3RαhiDC from mononuclear tonsillar cells, PBMC, and fetal lymph node (LN) cells, and on CD34+IL-3Rαhi cells from fetal bone marrow (BM)
| Tonsil | Fetal LN | PBMC | Fetal BM | |
|---|---|---|---|---|
| CD1a | − | − | − | − |
| CD3 | − | − | − | − |
| CD4 | ++/+++ | ++/+++ | ++ | ++ |
| CD5 | − | − | − | − |
| CD11b | − | − | − | − |
| CD11c | − | − | − | − |
| CD13 | −/+ | + | −/+ | + |
| CD14 | − | − | − | − |
| CD15 | − | ND | − | − |
| CD16 | − | − | − | − |
| CD19 | − | − | − | − |
| CD20 | − | − | − | − |
| CD32 | + | + | + | + |
| CD33 | + | +(+) | +(+) | +/++ |
| CD34 | −/+ | + | −/+ | ++/+++ |
| CD36 | ++/+++* | ++/+++ | ++/+++ | ++/++++ |
| CD40 | ++ | + | ++ | + |
| CD45RA | ++(+) | ++(+) | ++(+) | ++ |
| CD45RO | − | − | − | − |
| CD54 | ++/+++ | ND | ++/+++ | +/++ |
| CD56 | − | − | − | − |
| CD58 | + | ND | +/++ | +/++ |
| CD62L | − | + | +/++ | +++ |
| CD64 | − | − | − | − |
| CD80 | − | − | − | − |
| CD86 | + | ND | + | +(+) |
| HLA-DR | ++ | ++ | ++ | ++ |
| HLA-DQ | − | ND | −/+ | − |
Cells were stained as described in legends to Figs. 1, 3, and 4. Mean fluorescence intensity (MFI) levels for the IL-3Rαhi populations are expressed as −, indicating MFI in the first decade on a four log scale, which corresponds to isotype control levels. The +, ++, and +++ indicate MFI in the second, third, and fourth decades, respectively. A/sign means that MFI is on the border between two decades. Parenthesis means that the MFI is in the upper end of a decade. ND, not determined. The data are representative of at least three experiments.
In one out of three experiments tonsillar HLA-DR+lin−IL-3RαhiDC were negative for CD36.