Figure 1.
Phosphorylation of purified FKBP52 by CK2. Recombinant rabbit FKBP52 expressed in Sf9 cells (lanes 2, 4, 7, 9) or expressed in E. coli (lanes 3, 5, 8, 10) was incubated with (lanes 4, 5, 9, 10) or without (lanes 2, 3, 7, 8) CK2 in the presence of [γ-32P]ATP and analyzed by SDS/PAGE. Coomassie brilliant blue (CBB) staining (lanes 1–5) and autoradiography (lanes 6–10) are shown. As a control, CK2 was incubated without FKBP52 (lanes 1 and 6). The molecular masses (kDa) of markers are indicated on the left, and the position of FKBP52 is on the right. The positions of proteolytic fragments of FKBP52 are shown by arrowheads.