Figure 3.

Phosphorylation of various deletion mutants of FKBP52 by CK2. (A) Various deletion mutants of FKBP52 were phosphorylated in vitro by CK2. CBB staining is shown in the upper panel and autoradiography in the lower panel. Mutants are designated by their amino acid residue numbers on the top of lanes. The positions of wild-type FKBP52 and molecular mass markers (kDa) are shown on the left. The low molecular mass proteolytic fragments of FKBP52 are indicated by arrowheads. (B) Schematic representation of the phosphorylation of FKBP52 mutants by CK2. Mutants are designated by their amino acid residue numbers, and the degrees of the phosphorylation by CK2 are indicated on the right as follows: −, not at all phosphorylated; +, moderately phosphorylated; ++, strongly phosphorylated; and +++, very strongly phosphorylated.